Bulk culture of cell lines

The agency is looking to purchase a contract for the bulk culture of cell lines. Typically, we expect to require the bulk culture of two cell lines per year to about 1 x1010 cells each, with cell pellets provided at typically 1 x 108 cells, twice washed in PBS and snap-frozen in 50ml centrifuge tubes before storage at -80°C. Cells will typically be harvested at the end-point of cell cultures. Cell pellets will be sent to NIBSC on dry ice.