(NU/1671) The Supply and Installation of a Liquid Chromatography Mass Spectrometry (LC-MS) System with MS/MS Capability

Mass spectrometry-based proteomics has been extensively used in the field of cell biology and immunology, allowing the quantification of thousands of proteins as well as their modifications, localization, and dynamics for understanding cellular functions and processes on molecular level. Proteomics often provides a hypothesis-free and systems-wide characterisation of cellular or organellar proteomes. A standard proteomics workflow includes the following steps: extraction of proteins from cells or tissue, protein digestion by a protease into peptides, separation of peptides by liquid chromatography with online analysis of peptides by tandem mass spectrometry (LC-MS/MS), database-search for protein identification and software based quantification of the peptide ion intensities. This instrument will be for a successful mass spectrometry facility. We have in recent years successfully applied proteomics to a wide range of biological questions. We have analysed complex mammalian proteomes and sub-proteomes. Many of the samples are often only available in very small sample amounts and thus we require high sensitivity for our proteomics analyses. Moreover, we will use the tendered instrument for small molecule and metabolome analyses, requiring high resolution.